Table 4.
Real-time RTPCR results from Experiment 3
| Homecage | Restraint Duration Extension | Acute Restraint + Swim | Restraint History + Swim | Restraint History Only | Statistical analyses | |
|---|---|---|---|---|---|---|
| PVN | ||||||
| β-actin | 109.9 ± 14.6 | 106.8 ± 15.6 | 108.4 ± 12.0 | 111.3 ± 7.9 | 118.6 ± 8.25 | F(4,35) = 0.14, p > 0.05 |
| TNF-α | 103.5 ± 10.0 | 95.9 ± 9.7 | 76.0 ± 5.4 | 71.5 ± 8.6 | 87.6 ± 7.6 | F(4,33) = 2.39, p > 0.05 |
| IL-1β | 110.2 ± 18.7ab | 165.7 ± 49.8ab | 325.6 ± 67.8c | 194.4 ± 37.7b | 71.7 ± 7.0a | F(4,34) = 5.40, p < 0.01 |
| IL-6 | 101.6 ± 6.2 | 134.1 ± 27.0 | 127.3 ± 19.2 | 146.0 ± 24.1 | 100.7 ± 12.0 | F(4,34) = 1.16, p > 0.05 |
| c-Fos | 108.27 ± 18.1a | 224.2 ± 154.9d | 718.0 ± 26.5b | 555.0 ± 35.1c | 116.3 ± 6.7a | F(4,35) = 155.0, p < 0.001 |
| CD14 | 107.4 ± 16.6 | 114.3 ± 22.3 | 107.4 ± 16.6 | 105.6 ± 10.4 | 89.4 ± 5.4 | F(4,35) = 0.36, p > 0.05 |
| CD200 | 103.3 ± 8.7 | 103.4 ± 12.2 | 117.7 ± 7.7 | 118.0 ± 6.2 | 110.9 ± 5.6 | F(4,35) = 0.74, p > 0.05 |
| CD200R | 110.4 ± 16.6 | 95.4 ± 12.8 | 76.7 ± 11.7 | 87.4 ± 8.1 | 116.4 ± 10.1 | F(4,35) = 1.77, p > 0.05 |
| HPC | ||||||
| β-actin | 104.9 ± 10.5 | 111.9 ± 5.7 | 105.2 ± 7.6 | 109.2 ± 7.6 | 106.3 ± 7.8 | F(4,35) = 0.14, p > 0.05 |
| TNF-α | 103.1 ± 9.3 | 82.2 ± 6.8 | 97.9 ± 6.8 | 91.5 ± 11.2 | 92.5 ± 36.2 | F(4,35) = 0.65, p > 0.05 |
| IL-1β | 102.1 ± 8.2abc | 97.6 ± 4.4ac | 117.7 ± 16.4ab | 131.3 ± 12.5b | 86.7 ± 5.0c | F(4,35) = 2.88, p < 0.05 |
| IL-6 | 108.7 ± 17.6a | 148.4 ± 17.2ab | 195.8 ± 18.3b | 184.1 ± 23.9b | 125.1 ± 12.8a | F(4,35) = 4.14, p < 0.01 |
| c-Fos | 106.0 ± 14.4a | 111.4 ± 19.8a | 560.2 ± 42.3b | 458.6 ± 82.1b | 109.0 ± 8.69a | F(4,35) = 26.86, p < 0.001 |
| CD14 | 105.8 ± 14.7 | 116.9 ± 11.9 | 124.9 ± 10.5 | 128.8 ± 12.2 | 126.7 ± 13.4 | F(4,35) = 0.56, p > 0.05 |
| CD200 | 101.4 ± 6.6 | 132.2 ± 8.3 | 109.4 ± 5.9 | 115.7 ± 8.4 | 115.0 ± 6.6 | F(4,35) = 2.45, p > 0.05 |
| CD200R | 105.9 ± 15.5 | 107.4 ± 17.7 | 89.9 ± 9.1 | 79.2 ± 6.3 | 104.7 ± 11.2 | F(4,35) = 1.01, p > 0.05 |
| PFC | ||||||
| β-actin | 101.7 ± 7.3 | 89.2 ± 6.3 | 80.0 ± 12.9 | 91.2 ± 11.5 | 93.9 ± 8.8 | F(4,34) = 0.66, p > 0.05 |
| TNF-α | 109.9 ± 18.3a | 56.7 ± 7.9b | 65.0 ± 7.7b | 55.2 ± 6.9b | 77.4 ± 13.3ab | F(4,34) = 3.85, p < 0.05 |
| IL-1β | 110.2 ± 19.3a | 138.7 ± 9.0ac | 182.7 ± 11.2bc | 212.4 ± 22.1b | 131.1 ± 19.2a | F(4,35) = 6.02, p < 0.001 |
| IL-6 | 113.9 ± 19.0 | 117.6 ± 17.1 | 139.1 ± 14.1 | 130.4 ± 25.8 | 112.8 ± 21.2 | F(4,34) = 0.31, p > 0.05 |
| c-Fos | 109.1 ± 17.7a | 206.6 ± 18.8a | 1082.4 ± 67.2b | 814.8 ± 78.6c | 141.4 ± 9.9a | F(4,35) = 87.49, p < 0.001 |
| CD14 | 109.3 ± 16.5 | 85.6 ± 8.9 | 93.1 ± 9.2 | 86.9 ± 12.1 | 85.2 ± 8.2 | F(4,35) = 0.80, p > 0.05 |
| CD200 | 102.9 ± 8.8 | 87.8 ± 8.9 | 82.9 ± 12.2 | 82.8 ± 11.4 | 91.0 ± 8.7 | F(4,35) = 0.67, p > 0.05 |
| CD200R | 105.4 ± 11.7 | 108.7 ± 19.6 | 117.8 ± 12.8 | 78.9 ± 10.9 | 118.0 ± 15.0 | F(4,35) = 1.25, p > 0.05 |
Note. Means and SEM for Experiment 3. Gene expression data in the paraventricular nucleus of the hypothalamus (PVN), prefrontal cortex (PFC), and hippocampus (HPC) were adjusted to β-actin as a housekeeper and expressed as percent change from the control group (homecage). Bold text indicates a significant effect; data points marked with differing letters are significantly different from one another (p < 0.05).