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Evaluation of PikAIII-TE with a panel of truncated pentaketides 6–9. Enzymatic reaction conditions: 4 mM Pik pentaketide, 40 mM (10 equiv) MM-NAC, 20 mM (5 equiv) 2-vinylpyridine, 0.4 mM (10 mol %) NADP+, 10 mM (2.5 equiv) glucose-6-phosphate, glucose-6-phosphate dehydrogenase (2 units/mL), 4 μM (0.1 mol %) cell free PikAIII-TE, 8 h, stationary, RT.
