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. Author manuscript; available in PMC: 2018 Sep 27.
Published in final edited form as: J Am Chem Soc. 2017 Sep 19;139(38):13450–13455. doi: 10.1021/jacs.7b06432

Scheme 2.

Scheme 2

Reaction of PikAIII-TE with a panel of stereoisomer pentaketides 10–12. Enzymatic reaction conditions: 4 mM Pik pentaketide, 40 mM (10 equiv) MM-NAC, 20 mM (5 equiv) 2-vinylpyridine, 0.4 mM (10 mol %) NADP+, 10 mM (2.5 equiv) glucose-6-phosphate, glucose-6-phosphate dehydrogenase (2 units/mL), 4 μM (0.1 mol %) cell free PikAIII-TE, 8 h, stationary, RT.