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Evaluation of Pik TE with methyl protected hexaketides 26 and 27. Enzymatic reaction conditions: sodium phosphate buffer (400 mM, pH 7.2), 1 mM hexaketide, 8 mM 2-vinylpyridine, purified Pik TE (10 μM), 4 h, stationary, RT. Conversion of 26 to 28 and 27 to 32 was monitored (HPLC) with data represented as the mean ± standard deviation where n = 3.
