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. 2017 Sep 27;7:12347. doi: 10.1038/s41598-017-12564-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Linear absorption spectrum of the PSII RC as experimentally measured (black symbols) and theoretically calculated (blue line) together with the stick spectrum (purple columns) and the spectrum of the laser pulse (grey shaded area) used in the experiment at room temperature. (b) The circular dichroism spectrum as measured (black symbols) and calculated (red line) with the same parameters as used in (a). (c) Real part of the experimental (left) and theoretical (right) 2D photon echo spectra of the PSII reaction center at different waiting times. The theoretical result is calculated with the model parameters obtained by fitting the linear absorption and CD spectra.