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. 2017 Sep 27;7:12345. doi: 10.1038/s41598-017-12202-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of TGFβ Type-I and -II receptors by RT-PCR (A) and by Western bl.ot (B). (A) Expression of TGFβ Type-I and -II receptors in Taenia solium (Ts) and T. crassiceps (Tc) cysticerci. Amplified TGFβ Type-I and -II receptors obtained by RT-PCR from T. solium and T. crassiceps cysticerci are shown. A 50-pb ladder was use as DNA molecular Weight Marker; fragments amplified are 77 pb for TGFβ Type-I receptor, 170 pb for TGFβ Type-II receptor; actin beta-gamma (169 pb) was used as a positive control. (B) Western blot for the identification of putative TGFβ receptors in protein extracts from T. crassiceps cysticerci (T.c), T. solium (T.s), and human PMBCs (H), using polyclonal anti-human TGFβRI and TGFβRII antibodies. A rabbit anti-mouse IgG was included as a control. The white space in the human sample (H) for both TGFβ Type-I receptor TGFβ Type-II receptors mean that the blot was composed. The original blots are shown in Supplementary Figure 4.