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. 2017 Aug 24;6(3):39. doi: 10.3390/pathogens6030039

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Purification and characterization of Trypanosoma brucei lipid rafts. (A) T. brucei PF lipid rafts were prepared as described in Methods and subjected to Optiprep step gradient ultracentrifugation (diagrammed at top). Buoyant fractions containing lipid rafts floated to the top interface (red). Western blot analysis of fractions was performed using antibodies specific for the raft protein Calflagin (Tb44, Tb24 and Tb17) and the two fractions containing Calflagin (red) were used for proteomics; (B) Optiprep gradient fractions were analyzed by western blotting with positive control (Calflagin Tb44, CAP5.5) and negative control (mtHsp70) antibodies.