Figure 2.

Different types of DNA damage after a 1 h exposure to 5 mM AA, 6 Gy, 5 mM AA + 6 Gy and 500 μM H₂O₂. (A) Histograms of 8OH-dG lesions 48 h after treatments of LN18 cells. (B) Median fluorescence intensity (MFI) fold change compared with untreated cells of LN18, T98G, NZG0809 and NZG1003 cells. Values are averages ± SEM of at least 3 independent experiments in triplicate. Increased 8OH-dG lesions for H₂O₂ treatment over AA, Gy and AA + Gy was statistically significant (p < 0.05: unpaired two-tailed student t-test) for T98G and NZG1003. C. Representative photograph of comet tails (DSBs) of LN18 cells after a 1 h exposure to 5 mM AA. D. Fold change compared with controls of comet tail length of the different cell lines after a 1 h exposure to 5 mM AA, 6 Gy, 5 mM AA + 6 Gy compared to untreated controls (set at 1). Average number of comet tails measured per cell line: 278 (controls), 162 (AA), 220 (Gy) and 272 (AA + Gy).