Table 1. Optimized Parameters Determined by Fitting the Experimental Data to Langmuir and Multivalency Modelsa.
variant | KLM (M–1) | Kd,LM (nM) | Ceff (μM) |
---|---|---|---|
1H | 2.7 × 106 | 370 | N/A |
2H | 3.8 × 107 | 26 | 1.8 |
1 + 1H | 4.7 × 107 | 21 | 5.7 |
2 + 1H | 2.7 × 108 | 3.7 | 12 |
KLM is the apparent overall binding association constant (Kd,LM is the related binding dissociation constant) obtained from the fit using a 1:1 Langmuir-type model for each variant. Ceff is the effective concentration obtained from a fit using a multivalency model (see text and Methods).