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. 2017 Aug 29;11(9):9068–9083. doi: 10.1021/acsnano.7b03717

Table 1. Optimized Parameters Determined by Fitting the Experimental Data to Langmuir and Multivalency Modelsa.

variant KLM (M–1) Kd,LM (nM) Ceff (μM)
1H 2.7 × 106 370 N/A
2H 3.8 × 107 26 1.8
1 + 1H 4.7 × 107 21 5.7
2 + 1H 2.7 × 108 3.7 12
a

KLM is the apparent overall binding association constant (Kd,LM is the related binding dissociation constant) obtained from the fit using a 1:1 Langmuir-type model for each variant. Ceff is the effective concentration obtained from a fit using a multivalency model (see text and Methods).