Figure 5.
Sephadex G-100 size-exclusion chromatography profile of the sample eluted from Ni2+ Sepharose affinity chromatography (a). The chromatography was conducted with 5 mM ammonium acetate pH 6.8 buffer and 1 mL fractions were collected at a flow rate of 1 mL/min. Protein elution was monitored at 280 nm (___). The band profile in (12%) SDS-PAGE of the most representative fraction (numbered and marked with black triangles) is shown (b) along with the immunodetection of the purified rPoly p 5 using anti-His antibody (c).