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L. monocytogenes wt growth in HeLa cells (A) treated with BX-795 prior to infection and (B) transfected with a plasmid encoding (1) OPTN and TBK1 wt; (2) OPTN and TBK1 with an ineffective kinase (KM); and (3) an empty vector (CTRL). The phosphorylation of OPTN was confirmed by immunoblotting using antibodies against phosphorylated OPTN (OPTN-p), OPTN and β-actin (loading control). * p < 0.05 vs. CTRL.
