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Western blot analyses of Hbl B-specific IAC fractions of MHI 1532 supernatant: 1, supernatant of strain MHI 1532; 2, sample flow-through; 3, wash fraction; and 4, elution fraction. Toxin components were detected using the following mAbs: 1H9 (Hbl L2), 1E9 (Hbl L1), 1B8 (Hbl B), and 1E11 (NheB) ([20,33] and this study).
