Table 1.

Flow cytometry of Vero cells treated with rHbl B. Vero cells (1 × 10⁶) were incubated for 1 h with rHbl B (6.5 pmol/mL) and simultaneously with mAbs 1D7 (Hbl B-specific), 1D12 (Hbl B-specific) or 12D12 (Hbl B/L₁-specific) (ratio mAb:rHbl B = 1:1). Fluorescence was detected via Hbl B-specific Alexa Fluor^® 488-labelled mAb 1G8 (FL1, fluorescence at 488 nm).

Sample	FL1-Positive (%)
negative controls:
-	0.69 ± 0.29
1D7	0.96 ± 0.12
1D12	0.68 ± 0.03
12D12	0.53 ± 0.06
rHbl B and mAbs co-incubated:
rHbl B	41.95 ± 6.3
rHbl B + 1D7	96.37 ± 4.34
rHbl B + 1D12	43.99 ± 6.42
rHbl B + 12D12	50.81 ± 4.11
rHbl B and mAb 1D7 consecutively:
rHbl B, 1D7	32.76 ± 24.61
isotype controls:
rHbl B + IgG1	58.37 ± 7.33
rHbl B + IgG2a	46.36 ± 1.34