Table 2.
Proportion of Hbl components L2, L1 and B in sample flow-through, wash and elution fraction after IAC purification of B. cereus MHI 1532 supernatant. Hbl B-specific mAb 1B8 [29] and Hbl L2-specific mAb 1H9 (this study) were used for IAC. Hbl components were determined in sandwich EIAs: Hbl L2: mAbs 1A12 [32]/1H9-HRP (this study); Hbl L1: mAbs 1E9 [29]/1G8-HRP (this study); Hbl B: mAbs 1D12 (this study)/1B8-HRP [29]. NheB was also measured as a control (mAbs 2B11/1E11-HRP) [20]. EIA titres were multiplied with the respective sample volume resulting in units. The respective units determined in MHI 1532 supernatant were set to 100%.
| Hbl B-Specific IAC (1B8) | |||
| Sample Flow-Through | Wash Fraction | Elution Fraction | |
| Hbl L2 | 51% | 20% | 29% |
| Hbl L1 | <5% | <5% | 180% |
| Hbl B | <5% | <5% | 187% |
| NheB | 97% | - | 3% |
| Hbl L2-Specific IAC (1H9) | |||
| Sample Flow-Through | Wash Fraction | Elution Fraction | |
| Hbl L2 | 9% | 1% | 90% |
| Hbl L1 | 55% | 4.5% | 2% |
| Hbl B | 110% | 10% | 1% |