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. 2017 Jul 21;7(3):55. doi: 10.3390/biom7030055

Figure 5.

Figure 5

Semiquantitative expression of phospho-Tyr176-PTPIP51 (A), phospho-Ser46-PTPIP51 (B) and phospho-Ser212-PTPIP51 (C) in untreated HaCaT cells and in cells treated for 12 h with 5 µM, 50 µM and 100 µM LDC-3 (n = 3). The reaction intensities were recorded by ImageJ [22,23] and the resulting data were analyzed by GraphPad Prism 6 software (GraphPad Software, La Jolla, CA, USA). The significance of results were tested by Dunnett’s multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.