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Addition of Ni2+ induces rapid increase of thioflavin T (Tht) fluorescence. (A) Tht fluorescence kinetics of 17.64 μM Orb2A87 in the absence of metal (black) and in the presence of 2.5 molar excess of Ni2+ (cyan) and Mg2+ (pink). Buffer control with and without metals shows no fluorescence increase. The average and error of three technical repeats is shown; (B) Average of the Tht kinetics half times (T1/2) calculated from three independent repeats of the Tht time courses shown in (A).
