Figure 3.

Anticancer activity of Ad5 viruses after intravenous injection. Groups of 9–10 hamsters with subcutaneous HaK tumors were injected a single time with 1.5 × 10¹¹ vp of the indicated viruses and tumor sizes were measured. (A) Tumor size. Tumor dimensions were measured with calipers and tumor volume was calculated as width² × length × 1/2. The data are shown as mean ± standard error (SE). Tumor size statistics were calculated by a paired repeated measures T test between groups. Asterisks next to lines show significance between the group and the PBS group. Asterisks to the left of the legend with brackets represent the significant difference between Ad5-HB-ADP and other viral groups; (B) Effect of a single intravenous injection on survival. An animal lost survival when their tumor exceeded 2000 µL. Kaplan–Meier survival curves were analyzed by Log-rank (Mantel–Cox) test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.