Figure 1.

The representative 2D total correlation spectroscopy (TOCSY) spectrum from Mytilus edulis over 0–5 ppm. The spectrum is referenced to the chemical shift of TSP and the D₂O peak was removed. The box on the 2D plot connects the cross-peaks contributed by the resonances of the hydrogen atoms within alanine, where there is a doublet at 1.46 ppm and a triplet at 3.77 ppm. These coupling patterns are used to verify the identity of the compounds in Table 1; the complete list of all coupling partners generated from the TOCSY experiments is provided in Table S1.