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The FCLA assay for determining the accumulation of singlet oxygen using: (a) pristine Ce6; and (b) hybrid nanoparticles (Ce6-G4-UCNP) as the photosensitizer: (i) dark condition; (ii) 980; and (iii) 660-nm laser excitation; and (c) the Ce6-G4-UCNP with increasing loading capacity of Ce6 (0.43, 2.50, and 13.6 μM) under 980-nm laser excitation. The fluorescence intensity was normalized to the intrinsic fluorescence of FCLA solution in the absence of additives and light exposure.
