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. 2017 Sep 2;15(9):282. doi: 10.3390/md15090282

Figure 2.

Figure 2

Effects of 8 on protein expression levels of iNOS and COX-2 (a), and mRNA expression levels of IL-1β (Il1b) (b), IL-6 (Il6) (c), and TNF-α (Tnf) (d) in RAW264.7 macrophages. The cells were pre-treated for 3 h with the indicated concentrations of 8 and stimulated for 24 h (a), and 6 h (b–d) with LPS (1 μg/mL). The measurement of western blot analysis and RNA quantification for Il1b, Il6, and Tnf expression were performed as described in the Materials and Methods. Representative data from three independent experiments were shown. * p < 0.05 compared with the group treated with LPS.