Figure 3.

Effects of BDB on the binding of Nrf2 to ARE, Nrf2 transcriptional activity in UVB-exposed HaCaT cells, and GSS expression in siNrf2-transfected HaCaT cells. (a) Nuclear extracts were prepared and ChIP assay was performed to measure the binding of Nrf2 to ARE in the promoter of the GSS gene. Histone H3 was used the internal control; (b) Transcriptional activity of Nrf2 was measured using the ARE-luciferase reporter assay. *, # indicate significant differences from control and UVB groups, respectively (p < 0.05); (c) GSS expression was detected using Western blot analysis in control siRNA (siControl)- and Nrf2 siRNA (siNrf2)-transfected cells.