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Binding affinity of MP-A for TLR4, TLR2, and Dectin-1, as evaluated by surface plasmon resonance (SPR) analysis. Cumulative MP-A concentrations were injected at 30 μL/min over 60-second association periods, with the concentrations shown next to the arrows. (A,C,E) TLR4, TLR2, and Dectin-1 immobilization on CM5 sensor chips, respectively. (B,D,F) Kinetic-binding responses of MP-A to TLR4, TLR2, and Dectin-1, respectively.
