Figure 5.

ESMS reduced intracellular Ca²⁺ levels, increased ATPase activity and reduced CnA protein expression in H9c2 cells subjected to OGD: (A) the intracellular Ca²⁺ levels were evaluated by probe Fluo-3 AM. Bar is 40 μm (n = 3); (B) quantitative analysis of intracellular Ca²⁺ levels (n = 3); Na⁺-K⁺-ATPase (C), Ca²⁺-Mg²⁺-ATPase (D), and Ca²⁺-ATPase (E) activities were determined (n = 6); and (F) CnA protein expression was detected by Western blotting (n = 3). Results were presented as mean ± SD. TMZ = Trimetazidine dihydrochloride (positive control).