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. 2017 Aug 25;18(9):1825. doi: 10.3390/ijms18091825

Figure 7.

Figure 7

ESMS ameliorated the expression of mitochondrial mediated apoptosis proteins and reduced the caspase-3 activity in H9c2 cells subjected to OGD. (A) Bcl-2 and Bax expression were detected by Western blot (n = 3); (B) Caspase-3 protein expression was measured by Western blot (n = 3); (C) Mitochondrial fission was detected by Mito Tracker® Deep Red FM with a confocal laser scanning microscope. Bars are 40 μm for top, 10 μm for middle and 4 μm for bottom (n = 3); (D) Quantitative analysis of mitochondrial fission (n = 3); (E) Caspase-3 activity of all groups was evaluated (n = 6). Results were presented as mean ± SD. TMZ = Trimetazidine dihydrochloride (positive control).