Figure 7.

(A) Silver staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the supernatants of covalently bound and adsorbed diafiltrated tPA on SPION^Dex−COOH. The remaining tPA amounts in the supernatants of the reactions were analyzed with ImageJ to indirectly calculate the binding efficiencies; (B) The area of pure tPA’s bands were utilized for calculation of the respective binding efficiencies; (C) Binding efficiencies of covalently and non-covalently linked tPA to carboxymethylated iron oxide nanoparticles dependent on the tPA concentration. Shown are the mean values of n = 3 with standard deviations.