Figure 5.

HGF isoforms are differentially expressed in OMFs vs. DFs, and following supplementation with TGF-β₁. OMFs and DFs were transiently transfected with either siHGF or scrambled siRNA. siHGF- and scrambled siRNA-transfected cells were growth-arrested and maintained in serum-free F-SCM (open bars), or serum-free F-SCM containing TGF-β₁ (10 ng/mL, solid bars), for 72 h. (A) Differences in basal levels of full-length HGF and truncated NK1/NK2 isoform mRNA expression in OMF (black lines) and DF (grey lines) were analysed by RT-qPCR, and are displayed as 40-ΔCT. The expression of (B) full-length HGF, (C) truncated NK1, and (D) truncated NK2 mRNA, was assessed by RT-qPCR. Data is shown as mean ± S.E. for n = 3 independent experiments (* p ≤ 0.05, ** p ≤ 0.01; N/S, no significance).