Table 1.
Details of primers used for real-time polymerase chain reaction (RT-PCR), methylation-specific PCR (MSP), and chromatin immunoprecipitation (ChIP) analyses.
| Primer Name | Primer Sequence | Annealing Temp (°C) | Cycles | Length (bp) |
|---|---|---|---|---|
| CLDN6 | TTCATCGGCAACAGCATCGT | 58 | 35 | 345 |
| GGTTATAGAAGTCCCGGATGA | ||||
| DNMT1 | GAGGAAGCTGCTAAGGACTAGTTC | 56 | 30 | 141 |
| ACTCCACAATTTGATCACTAAATC | ||||
| SMAD2 | ATTTGCTGCTCTTCTGGCTCAG | 56 | 30 | 101 |
| ACTTGTTACCGTCTGCCTTCG | ||||
| SNAIL | GCCTAGCGAGTGGTTCTTCTG | 56 | 30 | 203 |
| TAGGGCTGCTGGAAGGTAAA | ||||
| E-cadherin | ATTCTGGGGATTCTTGGAGG | 56 | 30 | 337 |
| GGTCAGTATCAGCCGCTTTC | ||||
| N-cadherin | GTGCCATTAGCCAAGGGAATTCAGC | 56 | 30 | 370 |
| GCGTTCCTGTTCCACTCATAGGAGG | ||||
| Vimentin | AGCAGG AGTCCACTGAGTACCG | 56 | 30 | 200 |
| GTGACGAGCCATTTCCTCCTTC | ||||
| GAPDH | TGTTGCCATCAATGACCCCTT | 56 | 25 | 178 |
| CTCCACGACGTACTCAGCG | ||||
| CLDN6 U (MSP) | TGGATGTTTGTTAGTTTGAGGT | 58 | 35 | 500 |
| ATAACCACAACC CAAATTCACA | ||||
| CLDN6 M (MSP) | ACGTTTGTTAGTTCGAGGC | 58 | 35 | 502 |
| ATAACCGCAACCCGAATTC | ||||
| CLDN6 (BSP) | GAGGGGTAGAGATTTTGTTTTTGA | 53 | 30 | 210 |
| AATTAAATAAATTCCCCATATCACC | ||||
| CLDN6 (ChIP) | GCCACCTGGATGGCCGAGTC | 51 | 40 | 191 |
| GAGGGTTCCCAATTTGCGGG |