Figure 2.
Experimental approach to supply a patched hiPSC-CM with a synthetic inward rectifier potassium current (IK1). (A) Diagram of the experimental setup. The Real-Time Linux (RT-Linux)-based PC computes the synthetic IK1 according to the membrane potential Vm that is read into the PC. This IK1 is sent to the patch-clamp amplifier, operating in current clamp mode, which adds any stimulus current and injects the net current (Iinj) into the patched hiPSC-CM. This process is updated with a time step ∆t. (B) Current–voltage relationship of the synthetic IK1. EK is the potassium equilibrium potential.