Figure 4.

Variant splicing and dicing of human SphK2 isoforms. Schematic diagram of SphK2 variant isoforms based on mRNA and protein sequences acquired from GenBank. Colored boxes are representative of different RNA fragments or protein fragments, same color boxes are originated from the same origin of DNA sequences and are identical/similar sequences. The various described isoforms are derived from the expression of alternatively splicing SphK2 transcripts. Four protein isoforms have been identified for SphK2 and these express five SphK2 transcript variants. Human SphK2 isoform a (SphK2a/SphK-L) and SphK2 isoform b (SphK1b/SphK2-S) have been confirmed at the protein level. SphK2c and SphK2d protein translation has not been identified in humans to date. Variants X1-X5 were derived by automated computational analysis using gene prediction methods (Gnomon). Uniporter IDs are reported for variants SphK2 d, X5 and X6. All SphK2 sequences were aligned using Clustal Omega (V1.2.4) multiple sequence alignment (Available online: http://www.ebi.ac.uk/Tools/msa/clustalo/).