Figure 1.

Flower development and tepal pigmentation characteristics of Chinese narcissus cultivated variety “Jinzhanyintai”. (A) An overview of flower development of “Jinzhanyintai” after cultivated for 20 days in greenhouse. The sheathed leaves are robust and some flowers are opening; (B) Shoots with sheathed leaves come out from bulbs after planting for five days. This is the first time point for collecting tepals for RNA-seq (S1,T2); (C,G,H) Spathe with wrapped umbel (flower buds) was isolated from cultured bulbs five days after planting. The whole spathe and flower buds were completely embedded in shoots and displayed faint-yellow color (C,G); Tepals (T2) were collected and used for RNA-seq and metabolites analysis (H); (D,I,J) Spathe with wrapped umbel (flower buds) was isolated from bulbs 10 days after planting. Unopened spathes with wrapped umbel have emerged out from bulbs and turned deep-green (D,I); This was the second time point for collecting tepals for RNA-seq and metabolites analysis (S2,T3) (J); (E) New splited spathe with deep-green umbel (flower buds) isolated from bulbs 16 days after planting. The color of tepals at this time point (S3) was the same as in tepals at S2; (F) Umbel with opening flower and flower buds close to open after planting for 19 days. The color of tepals at this time point (S4) will gradually fade, turn from deep-green to green/white, and at last turn into pure white in opening flowers; (K,L) Green-to-white transition buds (K) and tepals (L). Tepals at this stage (T4) were collected and used for RNA-seq and metabolites analysis; (M,N) Opening Chinese narcissus flower (M) and its pure white tepals (N). Tepals at this stage (T5) were collected for RNA-seq and metabolites analysis. Bar = 1 cm.