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. 2017 Sep 8;18(9):1931. doi: 10.3390/ijms18091931

Figure 2.

Figure 2

Effects of TRIM44 knockdown on proliferation and motility of breast cancer cells. (A) Western blot analysis of TRIM44 knockdown efficiency in MCF-7 and MDA-MB-231 cells. Two kinds of siRNAs for TRIM44 (siTRIM44-A and -B) as well as siRNAs not targeting human transcripts (siControl-A and -B) were used. β-Actin protein was blotted as an internal control. IB, immunoblot. (B) Inhibitory effect by siTRIM44 on the proliferation of MCF-7 and MDA-MB-231 cells. MTS assay was performed at day five after transfection of siRNAs. Results are expressed as mean ± SEM (n = 4). *** p < 0.001 (two-way ANOVA). (C) Inhibitory effect by siTRIM44 on the motility of MDA-MB-231 cells. Cells were incubated for 24 h after transfection of siRNAs, and migration during the next 24 h was evaluated. Cells on the lower side of the filters were stained by the Giemsa stain solution and visualized under microscope at a magnification of 400×. Representative photographs of migrating are shown. The scale bars indicate 50 μm. (D) Cells migrating to the lower surface of the filters were counted in five fields. Results are expressed as mean ± SEM (n = 5). *** p < 0.001 (two-way ANOVA).