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Agonist activity assay. Caki1 cells were starved in serum-free medium for 24 h and incubated with 100 nM of c-Met antibodies or 50 ng/mL of HGF for 30 min. (a) Akt phosphorylation in Caki1 cells was measured by ELISA; (b) Western blot analysis of c-Met, phospho-c-Met, Akt, phospho-Akt, Erk1/2, phospho-Erk1/2, and β-actin.
