Skip to main content
. 2017 Sep 13;18(9):1968. doi: 10.3390/ijms18091968

Figure 5.

Figure 5

In vitro potency of IRCR201. All results are shown as the mean ± standard error of mean (SEM) from triplicate treatments. (a–d) Inhibitory effect of IRCR201 on cancer cell proliferation. MCF7, U87MG, MKN45, and A549 cells were treated with IRCR201, huOA5D5.v2, or human IgG control for 72 h. Cell proliferation was measured using CellTiter Glo® (Promega); (e) HGF-induced growth inhibition by IRCR201. The inhibitory effect of IRCR201 and huOA5D5.v2 on cell growth was examined under the condition of the addition of 50 ng/mL HGF in A549, an HGF-dependent cell. After 72 h of antibody treatment, the number of cells was measured with CellTiter Glo® (Promega); (f–i) Apoptosis assay. MCF7, U87MG, MKN45, and A549 cells were treated with IRCR201 or human IgG for 24 h. Apoptosis activity was detected with caspase-3/7 activity.