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RP-HPLC chromatograms obtained by the oxidation of R (10 µM) with 1 eq DHSox at pH 8.0 and 5 °C in the absence of EDTA. The reaction was acid-quenched after 0, 1, 5, 10, and 24 h. For HPLC analysis conditions, see the experimental section: (A) in the absence of CaCl2; (B) in the presence of 5 mM CaCl2.
