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. 2017 Sep 16;18(9):1996. doi: 10.3390/ijms18091996

Figure 5.

Figure 5

RP-HPLC chromatograms obtained by the oxidation of R (10 µM) with 1 eq DHSox at 5 °C in the presence of various metal ions. For HPLC analysis conditions, see the experimental section. (A) in the presence of 5 mM NaCl at pH 8.0 after 10 h; (B) in the presence of 5 mM KCl at pH 8.0 after 10 h; (C) in the presence of 5 mM MgCl2 at pH 8.0 after 10 h; (D) in the presence of 5 mM ZnCl2 at pH 6.8 (0.1 M Bis-Tris buffer) after 20 h; and (E) in the presence of 0.5 mM MnCl2 at pH 8.0 (60 mM Tris buffer) after 5 h.