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Oxidation pulse experiments. (A) RP-HPLC chromatograms obtained 0 to 180 s after addition of 1 eq DHSox to the reaction mixture, which was obtained by the reaction of R and 1 eq DHSox at pH 8.0 and 5 °C after 5 h. The oxidative folding conditions were the same as those of Figure 3B, but the reaction was quenched by AEMTS; (B) relative populations of R (●, blue), N (○, red), I-1 (▲, green), and I-2 (△, purple) as a function of the oxidation time.
