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Reduction pulse experiments. RP-HPLC chromatograms obtained three minutes after addition of indicated concentrations of DTTred to the reaction mixture, which was obtained by the reaction of R and 1 eq DHSox at pH 8.0 and 5 °C after 5 h. The oxidative folding conditions were the same as those of Figure 3B, but the reaction was quenched by AEMTS.
