Figure 8.

RP-HPLC chromatograms obtained by the oxidative folding of αLA using 1 eq DHS^ox at pH 8.0 and 35 or 45 °C in the absence or presence of CaCl₂. The initial concentration of R was 10 µM. The folding intermediates were trapped at different reaction times by acidification. (A) at 35 °C in the absence of CaCl₂; (B) at 35 °C in the presence of 5 mM CaCl₂; (C) at 45 °C in the absence of CaCl₂; (D) at 45 °C in the presence of 5 mM CaCl₂.