Figure 5.

Expression of VaSTS19 in Arabidopsis increases resistance to B. cinerea infection. (A) Representatives of three transgenic lines and a nontransgenic control line 3 d following B. cinerea inoculation; (B) average diameter of lesions on leaves of transgenic and nontransgenic lines, data are the means ± SD from three independent experiments (n = 30), asterisks indicate statistically significant difference between transgenic and nontransgenic lines (Student’s t test, ** p < 0.01); (C) symptoms of B. cinerea 3 dpi were scored by assigning lesions to one of three diameter classes: d ≤ 5 mm; 5 < d < 9 mm; d ≥ 9 mm, and three independent experiments were performed, each with at least 30 leaves; (D) transgenic and nontransgenic leaves were stained 3 dpi by trypan blue (a), DAB (b), and NBT (c) to observe the accumulation of dead cells and reactive oxygen, and the experiment was repeated three times, with 10 leaves each. Scale bars = 10 mm.