Figure 2.
Results of the atomic force microscopy (AFM) and live cell imaging measurements for LN229 and U87 (A) Shows the Young’s modulus. Statistical significance was found after irradiation of LN229 cells (nCTL = 60; n2Gy = 35) only. U87 (nCTL = 60; n2Gy = 35) cells did not show a change in elasticity; (B) Calculated mean adhesion energies using the Derjarguin-Muller-Topolov model. Irradiation led to a decrease in adhesion for both cell lines. The number of analyzed cells used for determination of adhesion energy were identical to those for the Young’s modulus; (C) Derived speeds from the time laps images. Inverse effects could be observed for the two cell lines. LN229 reacted with a decrease (nCTL = 118; n2Gy = 247), while irradiation of U87 led to an increase in cell speed (nCTL = 158; n2Gy = 118); (D) Regarding the contact area of the cells after irradiation both cell lines reacted with a decrease in area. The numbers of experimental values for the cell area were identical to the ones for the cell speed. The graphs show the mean value together with the standard error of the mean. Statistical analysis was performed using the Mann–Whitney test and significance was chosen for p < 0.05. The asterisk denotes significant results regarding the control measurement of the same cell line.