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The effect of Enhancer nanobody binding on the repeated photoswitching behavior of rsGreens in HeLa cells (500 cycles): (A) representative fluorescence photoswitching trace of rsGreen1, inset shows the initial five switching cycles; (B) signal of the maximally on-switched frame for each switching cycle scaled to the initial on-state fluorescence; and (C) baseline fluorescence (maximally off-switched frame) as fraction of the initial on-state fluorescence.
