Figure 6.

Effects of dual treatment (K252a + CQ) on cell proliferation, cell death and vasculogenesis in tumour tissue originating from mice SW480 and SW620 xenografts. After being engrafted subcutaneously by SW480 or SW620, mice were treated by K252a + CQ as described in Materials and methods section. Tumour sample were collected from five xenograft mice for both cell lines. (A) Proliferation was determined by Ki67 immunostaining. Magnification was ×100. (B) Necrosis was quantified using HES staining. Aeras of necrosis are delimited by N. Magnification was ×50. Results are representative of the ratio between necrosis area and total tumour area obtained with the Nanozoomer Digital Pathology 2.ORS (Hamamatsu) version 2.5.88 software. (C) Tumour sample were lysed and total protein were extracted. VEGF and cleaved caspase 3 were assessed by Western blotting. The density of each band was calculated with ImageJ software. Images show representative results performed for each condition, for both xenografts cell lines.