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. 2017 Apr 12;21(10):2503–2513. doi: 10.1111/jcmm.13171

Figure 4.

Figure 4

rhMG53 attenuated hepatic ischaemia/reperfusion injury via interaction with dysferlin. Rats were subjected to HIR or sham operation with or without administration of rhMG53 via caudal vein and then samples were collected after liver reperfusion for 6 and 24 hrs. (A and B) Immunofluorescence staining (×400) of liver dysferlin (DYSF) (Red) and nucleus (blue) and density analysis; (C) Western blot assay on liver dysferlin and MG53 at 6 hrs after reperfusion. (D) Immunoprecipitation assay of dysferlin/MG53 at 6 hrs after reperfusion. Date are mean ± SEM, n = 8/group, *P < 0.05, **P < 0.01. HIR, hepatic ischaemia/reperfusion; rhMG53, recombinant human MG53 protein.