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Sensitivity of the mPCR assay using different amounts of template genomic DNA of MTB H37Rv ranging from 100 ng to 1 pg per reaction analysed with 3% agarose gel‐electrophoresis. Primers for IS 6110 and npcTB_6715 were used at 0.1 μM and 0.5 μM, respectively. Ladder: 100 bp DNA ladder, ‐ve: Negative control.
