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. 2017 Apr 6;21(10):2491–2502. doi: 10.1111/jcmm.13170

Figure 3.

Figure 3

miR‐181‐5p suppresses HSCs activation through the direct targeting of Bcl‐2 and Stat3. (A) miR‐181‐5p potential binding sites on the 3′‐UTR of BCL‐2 and STAT3. (B) HST‐T6 cells were transfected with firefly luciferase transcript containing either a wild‐type or mutant form of 3′‐UTR of the two candidate genes, in the presence of either control or miR‐181‐5p, and then assessed for luciferase reporter activity at 48 hrs post‐transfection. The luciferase reporter activity of Bcl‐2 and Stat3 was suppressed by wild‐type miR‐181‐5p. (C) Protein expression of BCL‐2 and STAT3 was reduced by miR‐181‐5p in HST‐T6 cells. (D) mRNA expression of Bcl‐2 and Stat3 was reduced by miR‐181‐5p (E, F) Protein and mRNA expressions of BCL‐2 and STAT3 were reduced by exosomes derived from miR‐181‐5p‐modified ADSCs. **P < 0.01.