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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Nanomedicine. 2017 Apr 17;13(6):1925–1932. doi: 10.1016/j.nano.2017.04.004

Figure 2. Effect of mechanical injury on accumulation of peptides in the bladder.

Figure 2

A) Scheme of bladder injury of the apex via intravesical 24G catheter. Bladder was filled to distension with fluorescent peptide solution for 15 min and washed 5 times with PBS. Details of the procedure are provided in Methods. Images were acquired in situ through abdominal incision using Dino-Lite portable fluorescence microscope. Top image is fluorescence; bottom image is bright field; B) image of distended bladder filled with peptide solution; C) Images of bladders were taken in situ after peptide instillation and washing steps, but prior to bladder removal. There was no binding of CGKRK in the absence of focal injury, but efficient binding after the injury. KAREC showed limited binding in the apex (place of injury). There was no fluorescence in PBS treated bladders. All images were taken with the same exposure. Arrows point to the bladder apical region.