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. 2017 Sep 27;17:661. doi: 10.1186/s12885-017-3649-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Cell morphology followed by western blot and transwell invasion assays demonstrate that clusterin exert its influence in lung cancer epithelial-mesenchymal transition. A549 cells were treated with sCLU in concentration gradient. Cell morphology (a) and density (b) were significantly altered. Transwell invasion assays (Corning, USA) were applied to A549 cells treated with sCLU or BSA according to manufacturer’s instruction (c)