Figure 4.

Effect of EtOH-induced ALP activity and mineralized nodule formation in DPCs. (a, b) DPCs were treated with EtOH (50 mM) in the absence or presence of rapamycin (50 mM, pretreatment for 1 h). Cells were retreated every 3 days. ALP activity (a) and ALP mRNA expression (b) were measured at each time point. (c) DPCs were cultured in osteogenic induction medium for 14 days, and the mineralized nodule formation was assessed by von Kossa staining (scale bar = 100 μm). (d) On 14 days, the calcium content was determined. Data represent mean ± SD of 3 experiments with triplicates. ^∗p < 0.05 versus control group. ^∗∗p < 0.05 versus EtOH group.