Fig. 1. The flow cytometry assay indicates cholesterol exchange between live HeLa and U2-Os cells whereas no exchange occurs between Jurkat cells. Cells were treated with 5 μM bdp-cholesterol (1) for 18 h. Labelled and unlabelled live cells were co-cultured and flow cytometry was completed. The cell population was gated based on FSC-A vs. SSC-A (cell doublets were gated out using FSC-A vs. FSC-H) and histograms of mixed cells t = 0, 90 and 180 min are shown for each cell line with MFI (Mean Fluorescent Intensity) gated accordingly.
