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Protein expression in HG and H/R conditions. Western blotting for NLRP3 (a), ASC (b), procaspase-1 and caspase-1 (p10) (c), and IL-1β (d) in H9C2 cells. Data are expressed as the mean ± SD. n = 5 per group. ∗∗P < 0.01 versus N; #P < 0.01 versus LG; and ##P < 0.01 versus LG + H/R.